Interfacial Cholesterol Binding Sites   Anthony Lee - University of Southampton

To find word on page use Ctrl+F

Deep Cholesterol Binding Sites

• Introduction
• GPCR Class A
• Rhodopsin and Opsin
• GPCR Class B
• GPCR Class C
• GPCR Class F
• Adiponectin Receptor
• Ion Channels
• Porins Etc.
• Transporters
• Other Membrane Proteins

Interfacial Cholesterol Binding Sites

• Introduction
• GPCR Class A [Part 1]
• GPCR Class A [Part 2]
• GPCR Class B
• GPCR Class C
• GPCR Class F
• Adiponectin Receptor
• TRPA-TRPN channels
• TRPP-TRPV channels
• Potassium channels
• GABA_A receptors
• Scap-Insig dimer
• Dimer sterols

Interfacial Cholesterol Binding Sites on GPCRs

---

GPCRs Adiponectin Receptor

[With TM helix numbers; Units of E, kcals/mol with molar concentration units]

PDB	E	Local Residues
GPCR Adiponectin Receptor
Adiponectin Receptor, inactive, Human 3wxv
3wxv_EC	-13.7	L276[5], V279[5], T282[5], T286[5], F291, A294, T295, M300[6], G301[6], F304[6]
	-12.9	F145[1], L149[1], I153[1], M156[1], G174[2], L178[2], V181[2], L182[2], S185[2]
	-12.8	W302[6], I339[7], V342[7], L343[7], A346[7], F349[7], V350[7], Y353[7]
3wxv_IC	-14.9	Q265[5], T266[5], G269[5], V270[5], G273[5], L276[5], S277[5], V279[5], V280[5]
	-13.4	Q265[5], L272[5], L276[5], V279[5], I311[6], G315[6], A318[6], A319[6]
	-12.5	F304[6], A307[6], V308[6], I311[6], T312[6], L316[6], I321, F325
	-12.5	R122, F125, K126, I128, F129, F145[1], V146[1], L149[1]
Adiponectin Receptor, inactive, Human 5lwy
5lwy_EC	-13.2	Q179[2], V183[2], L186[2], L225[3], M228, V232[4], L250[4], I258, I261
	-13.1	L316[6], L320, L350[7], I353[7], F354[7], A357[7], F360, V361, H364
	-11.5	Q244[4], P245[4], I248[4], Y249[4], V252[4], L256[4], I291, H295
5lwy_IC	-13.1	G276[5], V277[5], G280[5], V281[5], G284[5], S288, I290, I291
	-12.8	R213[3], L214[3], K217[3], L218[3], S221[3], L225[3], I261, Q265[5]
Adiponectin Receptor, inactive, Human 5lx9
5lx9_EC	-13.1	L160, M167, L178[2], K181[2], V182[2], G185[2], F188[2], L189[2], I192[2], S196
	-12.2	V183[2], L186[2], L225[3], M228, V232[4], L250[4], C254[4], I258, I261
	-12.5	L316[6], L320, L350[7], I353[7], F354[7], A357[7], F360, V361, H364
5lx9_IC	-13.5	I139[1], F140[1], R141[1], I142[1], T146[1], W150[1], L154[1], W344[7]
	-13.1	L256[4], G276[5], V277[5], G280[5], V281[5], G284[5], S288, I291
	-13.1	G276[5], A279[5], G280[5], L283[5], G284[5], L287[5], I290, A329
	-12.2	L214[3], K217[3], L218[3], S221[3], L225[3], M228, I261, Q265[5]
	-12.0	V252[4], V255[4], L256[4], A259, V263[5], W266[5], V277[5], V281[5]
Adiponectin Receptor, inactive, Human 5lxa
5lxa_EC	-12.9	L316[6], L320, L350[7], I353[7], F354[7], A357[7], F360, V361, H364
	-12.7	Q179[2], V182[2], V183[2], Y236[4], F247[4], L250[4], I251[4], C254[4], V255[4], I258
5lxa_IC	-12.9	L189[2], L193[2], L214[3], K217[3], L218[3], S221[3], L225[3], I261, Q265[5]
	-12.0	V252[4], L256[4], V277[5], G280[5], V281[5], G284[5], S288, I291
Adiponectin Receptor, inactive, Human 5lxg
5lxg_EC	-14.4	Q168, V171[2], V172[2], M175[2], V221[3], Y225[3], L239[4], S240[4], C243[4], V244[4], I247[4]
	-13.6	V279[5], T282[5], M283[5], T286[5], G290, F291, A294, T295, M300[6], G301[6], F304[6], A307[6], I311[6]
	-12.7	W302[6], I339[7], V342[7], L343[7], A346[7], F349[7], V350[7], Y353[7]
5lxg_IC	-12.8	Y209[3], I212[3], I216[3], F220[3], F271[5], L274[5], G275[5], G278[5], F303[6], A307[6], Y310[6], I311[6], A314[6], Y317[6], F340[7]
	-12.7	M175[2], L178[2], L214[3], M217[3], G218[3], V221[3], I247[4], I250[4], I251[4], Q254[4], D256[4], R257
	-12.2	F271[5], L272[5], G275[5], L276[5], G278[5], V279[5], T282[5], A307[6], I311[6]
	-12.3	F145[1], L149[1], I153[1], L178[2], V181[2], L182[2], S185[2], F186[2], L189[2], F204[3]